I’m particularly interested in the temperature of the water we add, as in my limited reading (complete noob here), I’ve seen that in addition to the obvious effects of ambient temperature on the culture’s metabolism, the temperature of the water (particularly for the initial mix maybe?) can have a big effect. I’d like to start the little ones off with the best they can get, but I also understand the importance of uniform procedures. So I ask, does it matter for the experiment you’ve designed? I might just do one room temp and one closer to ideal just for my own edification, but it’d be good to know if the experiment requires the room temp for good data! (Q from Nathan Staffa)
LMN: Really good points! This is what we love about this: we could do a whole separate experiment just to explore these questions! Given our directions, we assume most will use room temperature water. SO using room temp water will give us the best comparison to other’s data. But, if you want to use slightly warmed water, you can do that. Make sure to note it in the “other notes” section when you enter your data. But making two starters, one with warmed water and one with room temp water, would be really neat! We would certainly be interested in the results.