LMN: Really good points! This is what we love about this: we could do a whole separate experiment just to explore these questions! Given our directions, we assume most will use room temperature water. SO using room temp water will give us the best comparison to other’s data. But, if you want to use slightly warmed water, you can do that. Make sure to note it in the “other notes” section when you enter your data. But making two starters, one with warmed water and one with room temp water, would be really neat! We would certainly be interested in the results.